CAS NO:333338-18-4 PNPP/4-Nitrophenyl Phosphate Disodium Salt Hexahydrate

CAS NO:333338-18-4 PNPP/4-Nitrophenyl Phosphate Disodium Salt Hexahydrate

Molecular Formula:C6H9NNaO7P
Molecular weight:261.1
Application:pNPP(Hexahydrate) is a chromogenic substrate for phosphatase, mainly used in biochemical research, ELISA immunoassay and in vitro diagnostic reagent industries.
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Description
Technical Parameters

 

Products Description

 

pNPP(Hexahydrate) hexahydrate has good water solubility. It hydrolyzes to produce yellow soluble product detectable at 405 nm by spectrophotometry. It features high sensitivity, stable color development, reaction stoppable by alkaline solution and excellent quantitative repeatability.

 

Products  Specification

 

Product name pNPP/4-Nitrophenyl phosphate disodium salt hexahydrate
CAS NO 333338-18-4(Hexahydrate)
ITEM NO M314
Appearance White to pale yellow crystalline powder
Purity ≥99%
Melting point >300 °C
Package 100mg/1g/100g etc.
Delivery 2-3days
Storage -20°C
MSDS/COA/TDS Connect us

 

Products Application

 

 

 

 

product-1000-833

1.In vitro diagnostic industry: Chromogenic substrate for ELISA kits, immunoassay with alkaline phosphatase label, clinical serum sample detection.

2. Biochemical research industry: Determination of alkaline phosphatase and acid phosphatase activity, enzyme kinetics and enzyme inhibitor screening.

3.Life science research: Tracking phosphatase activity during protein purification, basic enzymology research.

 

Megma also offers a color-developing substrate product for alkaline phosphatase (ALP):

PNPP (Anhydrous)

CAS 4264-83-9

 

FAQ

 

Q: How to store pNPP 4-Nitrophenyl phosphate disodium salt hexahydrate?

A: Seal powder, protect from light and store dry at -20℃. It is sensitive to light and moisture to prevent premature hydrolysis.

Q: What about the stability of its aqueous solution?

A: Prepare freshly before use. The aqueous solution is prone to hydrolysis and degradation under light, long-term storage is not recommended.

Q: Could it be used for ELISA kit manufacturing?

A: Yes, high-purity pNPP is classic alkaline phosphatase chromogenic substrate for ELISA and widely used as raw material for in vitro diagnostic kits.

Q: What should be noted when weighing anhydrous pNPP (CAS4264-83-9) and hexahydrate pNPP (CAS333338-18-4) for solution preparation?

A: They have different molecular weights. The anhydrous form has a smaller molecular weight, so less mass is needed for the same molar concentration. They cannot be substituted by the same mass when preparing buffer substrate solutions.

 

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product-3500-1166

 

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