Products Description
pNPP(Hexahydrate) hexahydrate has good water solubility. It hydrolyzes to produce yellow soluble product detectable at 405 nm by spectrophotometry. It features high sensitivity, stable color development, reaction stoppable by alkaline solution and excellent quantitative repeatability.
Products Specification
| Product name | pNPP/4-Nitrophenyl phosphate disodium salt hexahydrate |
| CAS NO | 333338-18-4(Hexahydrate) |
| ITEM NO | M314 |
| Appearance | White to pale yellow crystalline powder |
| Purity | ≥99% |
| Melting point | >300 °C |
| Package | 100mg/1g/100g etc. |
| Delivery | 2-3days |
| Storage | -20°C |
| MSDS/COA/TDS | Connect us |
Products Application

1.In vitro diagnostic industry: Chromogenic substrate for ELISA kits, immunoassay with alkaline phosphatase label, clinical serum sample detection.
2. Biochemical research industry: Determination of alkaline phosphatase and acid phosphatase activity, enzyme kinetics and enzyme inhibitor screening.
3.Life science research: Tracking phosphatase activity during protein purification, basic enzymology research.
Megma also offers a color-developing substrate product for alkaline phosphatase (ALP):
|
PNPP (Anhydrous) |
CAS 4264-83-9 |
FAQ
Q: How to store pNPP 4-Nitrophenyl phosphate disodium salt hexahydrate?
A: Seal powder, protect from light and store dry at -20℃. It is sensitive to light and moisture to prevent premature hydrolysis.
Q: What about the stability of its aqueous solution?
A: Prepare freshly before use. The aqueous solution is prone to hydrolysis and degradation under light, long-term storage is not recommended.
Q: Could it be used for ELISA kit manufacturing?
A: Yes, high-purity pNPP is classic alkaline phosphatase chromogenic substrate for ELISA and widely used as raw material for in vitro diagnostic kits.
Q: What should be noted when weighing anhydrous pNPP (CAS4264-83-9) and hexahydrate pNPP (CAS333338-18-4) for solution preparation?
A: They have different molecular weights. The anhydrous form has a smaller molecular weight, so less mass is needed for the same molar concentration. They cannot be substituted by the same mass when preparing buffer substrate solutions.
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