CAS NO:39450-01-6 Proteinase K

The core function is to specifically degrade various protein impurities in the sample, while inactivating nucleases and eliminating the risk of protein encapsulation and degradation of nucleic acids. It can effectively protect the integrity of nucleic acids. This is fundamentally attributed to the triple key characteristics of substrate specificity, resistance to denaturants, and absence of nucleic acid hydrolytic activity. It fundamentally solves the two core problems in nucleic acid extraction, namely "protein encapsulation of nucleic acids and nucleic acid degradation by nucleases".

The main purpose is to degrade various protein impurities in the food matrix, break the encapsulation and adsorption of proteins on nucleic acids, release the target nucleic acids (transgenic fragments) in the samples, at the same time inactivate endogenous and exogenous nucleases in the food, eliminate the risk of nucleic acid degradation, improve the yield, purity and integrity of subsequent nucleic acid extraction, and ensure the sensitivity and accuracy of transgenic PCR / fluorescence quantitative PCR detection. Its heat-resistant agent and broad-spectrum protein hydrolysis properties can be adapted to various complex food matrices in food detection, such as those with complex components, high protein content and strong matrix interference.

The core concept of the proteinase K reaction system is to first use physical/chemical methods to break the dense cross-linking structure of keratin, and then adapt the optimal catalytic conditions for proteinase K, thereby enhancing the contact efficiency between the enzyme and the substrate. Using proteinase K alone results in extremely low hydrolysis efficiency.

1. Nucleic acid extraction: removing nucleases and other protein contaminants;
2. Biopharmaceuticals: DNA vaccines, heparin, etc. for the preparation of non-protein biological products;
3. Industrial fields: tanning, cosmetic raw materials, and feed processing;
4. Research purposes: protein digestion, key components of genomic DNA/RNA extraction kits;
5. This enzyme has broad-spectrum protein hydrolysis ability. Its stability and efficiency make it a routine laboratory tool.

This is a non-specific serine protease used for protein decomposition. In the presence of sodium dodecyl sulfate (SDS) or urea, proteinase K is active at different pH values (4 - 12), salt concentrations, and temperature units.

1. Wear a dust mask or N95 mask, chemical safety goggles, and nitrile/latex gloves.
2. It should be carried out in a fume hood to avoid generating dust.

3. After the operation, wash hands thoroughly.